sharpen_map#

summary#

Sharpen a map by flattening the power spectrum of the high resolution Fourier amplitudes, optionally adding a b-factor.

Can apply statistics calculated from calculate_fsc.

Optionally invert handedness of the map.

cli options#

none

interactive options#

input_volume

  • Name of input image file.

output_volume

  • Name of sharpened output volume.

input_mask

  • Name of input image file.

res_statistics

  • Input reconstruction statistics, The table listing FSC, Part_FSC, Part_SSNR and Rec_SSNR.

use_statistics

  • Use statistics”, “Answer No if no statistics are available?.

pixel_size

  • Pixel size of the map in Angstroms.

inner_mask_radius

  • Inner radius of mask to be applied to the input map, in Angstroms.

outer_mask_radius

  • Outer radius of mask to be applied to the input map, in Angstroms.

bfactor_low

  • B-factor to be applied to the non-flattened part of the amplitude spectrum, in Angstroms squared.

bfactor_high

  • High-res B-Factor (A^2). B-factor to be applied to the flattened part of the amplitude spectrum, in Angstroms squared.

bfactor_res_limit

  • Low resolution limit for spectral flattening (A). he resolution at which spectral flattening starts being applied, in Angstroms. (Default: 8.0)

resolution_limit

  • High resolution limit (A). Resolution of low-pass filter applied to final output maps, in Angstroms. (Default: 3.0)

filter_edge

  • Filter edge width (A). Cosine edge used with the low-pass filter, in Angstroms. (Default: 20.0)

fudge_SSNR

  • Part_SSNR scale factor. Scale the Part_SSNR curve to account for disordered regions in the map. (Default: 1.0)

use_mask

  • Should the 3D mask be used to mask the input map before sharpening?.

invert_hand

  • Invert handedness. Should the map handedness be inverted?